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Oncol 130 WatsonJ

The inherent pathological changes of stroke have been shown to be closely related to the characteristics of ferroptosis, including iron metabolism disorders, lipid peroxidation, and elevated ROS levels

PMID: 24791752

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A Shimadzu LC20AD, Ultra Performance Liquid Chromatography (UPLC) system (Shimadzu Corporation, Kyoto, Japan) equipped with Shimadzu 8040 triple quadrupole (QqQ) mass analyzer (Shimadzu Corporation, Kyoto, Japan) with an electrospray ionization source (ESI) operated in the positive charge mode for the quantification of 2

For experiments requiring manipulation of cystine availability, cells were cultured in RPMI 1640 medium without L-glutamine, L-cysteine, L-cystine, and L-methionine (Fisher Scientific, ICN1646454) supplemented with 5% dialyzed FBS (Sigma-Aldrich, F0392), L-glutamine (VWR, VWRL0131-0100), and L-methionine (Sigma-Aldrich, M5308)